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Category Icon RC-30 (13)
Category Icon RC-30HV (12)
Category Icon RC-30WA (13)
Category Icon RC-31 (7)
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There were 21 questions found in this category:
  1. questionCan the RC-21B/RC-21BR be used on a confocal microscope?
    Yes, the RC-21B and RC-21BR can be used on a confocal microscope. In general, one critical consideration when using imaging and recording chambers in confocal imaging is the small working distance of the objective. All Warner chambers use a #1 coverslip as the chamber floor which should allow vi ...

  2. questionCan the RC-30WA function as a stop-flow device?
    The term 'stop-flow' assumes there will be a perfusion system in place, but the flow is intended to stop periodically. First, we've never specifically tested the chamber as a stop-flow device and so have not quantitated how the solution comes to rest. We have made many informal measurements with ...

  3. questionCan the TSP wash remove vacuum grease from plastics?
    The short answer is that the TSP wash procedure will not remove vacuum grease from plastic. Your best option is to remove the grease by mechanical means (e.g., rub it off with a Kimwipe). You should then be able to clean things up using the TSP wash.

  4. questionHow can a steel Sutter stand be insulated from a heated series 20 chamber?
    When heating a sample in a chamber an insulator can be used to prevent the steel Sutter stand from acting as a heat sink. The insulator can be made out of a thin layer of plastic, cardboard, or even a latex glove.

  5. questionHow can I generate and maintain a hypoxic solution for delivery to a chamber?
    This is normally achieved by bubbling an O2-free gas through the solution until the O2 load has been depleted. Keep in mind, however, that PE, Tygon, and Teflon tubing are all gas permeable, and therefore will expose your perfusing solutions to O2 during transit.One option to address this is to ...

  6. questionIs it possible to use temperature feedback from the bath to regulate an inline solution heater?
    Unfortunately, no. Feedback in inline solution heaters is derived from thermistors located within the heater body. This prevents oscillations in temperature which can arise when the temperature sensor is distal to the heat source..

  7. questionIs the RC-30HV tested at different flow rates and if so, what is the highest flow at which the liquid stays in the channel?
    While we have measured the flow rate in the RC-30 to upwards of 20 ml/min, these chambers typically support flow rates from 0.1 to 2.0 ml/min. This lower flow range is not due to physical limitations of the chambers but is more reflective of the experimental conditions.. Many researchers speak o ...

  8. questionIs the RC-31 compatible with the TC-324B temperature controller?
    Yes. You will also need a CC-28 cable.

  9. questionWhat bath geometries are available with the RC-30 series confocal imaging chambers?
    Pre-cut gaskets are supplied with the RC-30 series chambers; one cut wide to allow full use of the aperture window and a narrow, slotted version for fast solution exchange. A blank gasket, that has not been pre-cut, is also provided to allow for a user-defined geometry.

  10. questionWhat do I do if my RC-30 confocal imaging chamber leaks?
    The gaskets that are used with the RC-30 confocal imaging chamber come in three thicknesses: 150, 250 and 375 um. Leaking is not uncommon when only a single150 um gasket is used. This is because the thinnest gasket allows little compression. We suggest using either a 250 um gasket or two 150 um ...

  11. questionWhat is the bath volume of the RC-30 Series gaskets?
    Gaskets come in 3 varieties, slotted, wide-bath, and blank. For a 250 um thick gasket, the slotted and wide-bath hold 26 and 133 ul, respectively. Since the blank is designed to allow the creation of a custom bath geometry, you'll need to calculate that volume independently.

  12. questionWhat length of electrode is exposed to the bath in your field stimulation chambers?
    Chamber Exposed Electrode (on each side of bath)RC-21BRFS 12mmRC-27NE 25mmRC-37FS 19mmRC-37WS 13mmRC-47FSLP 15mm

  13. questionWhat thickness gaskets are available for use with the RC-30 series confocal imaging chambers?
    Gaskets for the RC-30 series chambers are available in three thicknesses; 100uM, 250uM and 375uM.

  14. questionWhen is in-line heating preferred over chamber heating?
    Generally speaking, heating a platform alone is a good strategy only for very slow or non-flow conditions. The solution loses most of its heat enroute to the chamber. Under the more common flow conditions of 2-10 ml/min, an inline heater is a better way to deliver heated solution to a chamber.Co ...

  15. questionWhere should the thermistor be placed in the RC-30HV chamber?
    Because the RC-30HV is a closed bath chamber, it is necessary to put the small, monitor thermistor into the chamber's outflow stream. One method is to put a small tee fitting in the outflow line and insert the thermistor into the open port. Seal around the thermistor with silicone grease or syl ...

  16. questionWill an Series 20 chamber leak if it gets scratched?
    It depends on how deep the scratch is! Clearly, a cut cannot be so deep that the vacuum grease cannot seal the chamber. In addition, if scratches are too broad or are too numerous, then there will not be sufficient compression on the coverglass to get a tight seal and the chamber could leak. Th ...

  17. questionAre coverslips appropriate for cell culturing?
    Coverslips are appropriate for cell culture but are not coated. They will, however, accept Cell-Tak and poly-L coatings if needed.

  18. questionAre the gaskets used in the RC-30 Chambers easy to cut?
    The gaskets are very easy to cut as long as you work on a hard surface and use a sharp blade.

  19. questionCan a PDMS microfluidic device be used in lieu of gaskets with an RC-30 Chamber?
    A thin layer of PDMS works very well as long as the upper surface is kept flat during fabrication. (We've tried it!) This statement assumes the chamber is built onto the bottom coverslip.

  20. questionDon't see your answer? Please submit a question!
    We welcome your questions! Submit a question to us (via email) by clicking here and we'll answer you directly. We'll also anonymously post your question to our FAQ Knowledge Base (KB) to assist other people.If you choose to not communicate via this KB, then please send your questions directly to ...

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