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Perfusion Cups
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There were 8 questions found in this category:
Can strong acid solutions (pH 1) be used with bilayer cups and chambers?
No. Bilayer chambers are made of Delrin with a polycarbonate window. The Delrin is resistant but the window is not.Bilayer cups are made from Delrin, polystyrene, or polysulfone. Again, the Delrin is resistant but the polystyrene and polysulfone are not.
Does Warner make Teflon bilayer cups?
All Warner bilayer chambers are made from Delrin. Delrin has the same chemical properties of Teflon but has greater physical durability. Warner bilayer cups are made from Delrin, polystyrene, or polysulfone.
How long can you use an agar bridge for?
You can use an agar bridge until it fails (days to months). Failure mode is usually expressed as an increase in bridge leakiness. Many researchers replace bridges daily which minimizes bactrial/fungal growth or the introduction of other contaminants.
Is sodium phosphate dibasic a suitable substitute for sodium phosphate tribasic when cleaning bilayer cups and chambers?
While the tribasic salt (TSP) is the clearly effective, a dibasic salt (DSP) can also work if the concentration is increased slightly. The monobasic salt, however, is pretty much useless. My best recommendation is to not waste your time with the dibasic or monobasic salts and go directly to the ...
What cuvette material is most suitable for permanent use?
The polystyrene and Delrin cups are the most popular. We also offer cups in polysulfone.Polystyrene yields better holes but is less chemically resistant than Delrin. Polysulfone was introduced as a complormise between polystyrene and Delrin. All cup materials should last for a minimum of 6 month ...
What type of cup material will allow a thick Bilayer membrane to form?
The thickness of a bilayer membrane is primarily determined by the lipid tail length and partially determined by the solvent. Therefore, you should expect the membrane thickness to be the same regardless of the cup material. You may experience a differenct membrane capacitance on cups of diffe ...
At what aperture size is the bilayer most stable?
The choice of aperture size depends the science. The constraints revolve around the lipids used, the difficulty in incorporating a channel, and the channel's kinetics. Generally, speaking the smaller the hole, the lower the capacitance noise in the system. The result of which is the ability to r ...
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