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Chamber Systems
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Classic, Self-contained, NaviCyte, EasyMount
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There were 7 questions found in this category:
 How do I know what Ussing System components and accessories I will need?
The Ussing System brochure does a good job of making sure you don't miss anything as you put a system together. Please see the PDF.
- What are the differences between round and slotted inserts?
Round and slotted inserts can be interchanged provided the tissue is large enough. Usually, a slotted insert is employed for a small diameter intestinal section. The extra length increases the surface area while accommodating the small width.
- What are the top and bottom volumes of the NaviCyte horizontal ussing chamber?
The volume of the basolateral (lower) compartment is 1.5 ml. The volume of the apical (upper) compartment is 1.5-2.0 ml, depending on if electrodes are used, and if the tissue ring or Snapwell culture cups is used.1.5 ml applies for the case of a tissue ring without electrodes, and 2.0 ml applie ...
- What is the content of the 69-0987 electrode set?
The P2020 Electrode Set (cat# 69-0987) includes 2 sintered Ag/AgCl pellet electrodes for voltage sensing (black), two Ag wire electrodes for current passing (white) and 8 electrode tips.
- Which Sylgard product is recommended for use with Ussing chambers?
The most common version used in electrophysiology is Sylgard 184. Several vendors can be found with a Google search of the full name.
- Why would I select pins over o-rings in an Ussing System?
If the basement membrane is strong enough, then pins can be used. It is easier to stretch the membrane over the hole with pins.If the tissue is more fragile, then you must use o-rings. The tissue can be held into place during assembly by placing a small amount of vacuum grease around the o-ring.
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We welcome your questions! Submit a question to us (via email) by clicking here and we'll answer you directly. We'll also anonymously post your question to our FAQ Knowledge Base (KB) to assist other people.If you choose to not communicate via this KB, then please send your questions directly to ...
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