While we have measured the flow rate in the RC-30 to upwards of 20 ml/min, these chambers typically support flow rates from 0.1 to 2.0 ml/min.  This lower flow range is not due to physical limitations of the chambers but is more reflective of the experimental conditions.. 

Many researchers speak of the 'rule of 5' or the 'rule of 10' for solution exchange in a chamber containing cultured cells.  The 'rule of five' means that exchanging the chamber volume 5 times per minute will maintain the desired nutrient and O₂ load for a particular cell line or tissue. 

Therefore, if the chamber volume is 133 ul (i.e. ~0.15 ml) then the 'rule of 10' says that a flow rate of 1.5 ml/min is sufficient to replenish that volume 10 fold every minute.