First, look at page 25 in the BC-535 manual for a brief introduction.

As a start, I've had good results using a 5:3 ratio of PE:PC at 30-50 mg/ml in decane. If your lipids came lyophilized, then they will need to be brought up in chloroform to solubilize them. Then, aliquot the desired ratio into a culture tube and dry using nitrogen or argon. Finally, re-solubilize the lipids with decane. The important part here is to completely evaporate the chloroform or the membranes will be fragile!

Next, precoat the hole using your decane/lipid mixture and assemble your cup/chamber with buffer and salt bridges in place.

Place the assembly into your Faraday cage and make your electrical connections. Next, verify that you have an open hole and zero the offset potential. Finally, you are positioned to form a membrane. You can use a glass rod, paintbrush, or the bubble method.